Mario raced down the Rainbow Road and teamed up with Donkey Kong in the final trailer for released on Thursday by Universal Pictures. 
The 95-second trailer opened ominously with a panoramic view of Bowser Castle with hot lava flowing from the volcanic home of the Koopa villain's stronghold.
Luigi [Charlie Day] was next shown imprisoned in a hanging cage inside Bowser Castle.
'Where am I?,' Luigi asked in a fearful voice.
'Oooh, fresh meat for the grinder,' said a blue caged Lumas.
Final trailer: Mario raced down the Rainbow Road to save his brother Luigi in the final trailer for The Super Mario Bros.

Movie released on Thursday by Universal Pictures
Another prisoner advised Luigi to ignore him.
'There's gotta be a way outta here,' Luigi said while looking around frantically.
'There's no escape, the only hope is the sweet relief of death,' the Lumas said.
'Whoa,' the other prisoners exclaimed at his morbid outlook.
Bowser [Jack Black] then amassed his army.
'We will destroy the Mushroom Kingdom!,' Bowser vowed.
Princess Peach [Anya Taylor-Joy] warned everyone that Bowser was coming as Mario declared that he wasn't afraid.
Mario and polka dot chocolate Donkey Kong [Seth Rogen] were then shown teaming up as they smashed obstacles together.
Dynamic duo: Donkey Kong and Mario teamed up together during the trailer
Ominous opening: The 95-second trailer opened ominously with a panoramic view of Bowser Castle with hot lava flowing from the volcanic home of the Koopa villain's stronghold
Encaged prisoner: 'There's gotta be a way outta here,' Luigi said while looking around frantically
No escape: 'There's no escape, the only hope is the sweet relief of death,' the Lumas said
Princess Peach jumped on a motorcycle while Mario climbed into a cart before they sped onto the Rainbow Road.
Mario got creative on the colorful roadway while fighting off villains at high speed.
The voice cast also includes Keegan-Michael Key as Toad, Fred Armisen as Cranky Kong, Sebastian Maniscalco as Foreman Spike and Kevin Michael Richardson as Kamek.
The Super Mario Bros.

Movie was produced by Universal Pictures through its Illumination animation division and will be in theaters on April 5.
Leading voice: Chris Pratt, shown last month in Los Angeles with wife Katherine Schwarzenegger, provides the voice of plumber Mario
Invasion plan: 'We will destroy the Mushroom Kingdom!,' Bowser vowed
Cart driving: Mario got creative on the colorful roadway while fighting off villains at high speed
Coming soon: The Super Mario Bros.

Movie was produced by Universal Pictures through its Illumination animation division and will be in theaters on April 5